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anti ha polyclonal antibody  (Proteintech)


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    Structured Review

    Proteintech anti ha polyclonal antibody
    Anti Ha Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1639 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+ha+polyclonal+antibody/GFP+tag+Antibody/pm41910148-174-72-75
    Average 96 stars, based on 1639 article reviews
    anti ha polyclonal antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Glycosylated NS3/NS3A protein of bluetongue virus facilitates efficient viral egress via lipid raft anchoring.
    Article Snippet: .. Commercial antibodies used in this study included anti-FLAG (DYKDDDDK) monoclonal antibody (1:1,000 for immuno fluorescence assay [IFA], 1:10,000 for western blotting [WB]; 66008-4-Ig, Proteintech), anti-HA polyclonal antibody (1:100 for IFA, 1:1,000 for WB; 51064-2-AP, Proteintech), anti-β-actin monoclonal antibody (1:10,000 for WB; 66009-1-Ig, Proteintech), anti-Cal nexin polyclonal antibody (1:200 for IFA; 10427-2-AP, Proteintech), anti-Syntaxin 6 polyclonal antibody (1:200 for IFA; 10841-1-AP, Proteintech), anti-Flotillin 1 monoclonal antibody (1:100 for IFA; 67968-1-Ig, Proteintech), and anti-Filamin A (FLNA) monoclonal antibody (1:1,000 for WB; 67133-1-Ig, Proteintech). .. Secondary antibodies included Alexa Fluor 488- or 568-conjugated goat anti-mouse/rabbit IgG (H+L) highly cross-adsorbed antibodies (1:1,000; Invitrogen), Wheat Germ Agglutinin Alexa Fluor Plus 488 (1:1000; W11261, Invitrogen), and HRP-conjugated goat anti-rabbit and anti-mouse IgG (1:5,000 for WB; 31,460 and 62-6520, Thermo Fisher).

    Immunofluorescence:

    Article Title: Glycosylated NS3/NS3A protein of bluetongue virus facilitates efficient viral egress via lipid raft anchoring.
    Article Snippet: .. Commercial antibodies used in this study included anti-FLAG (DYKDDDDK) monoclonal antibody (1:1,000 for immuno fluorescence assay [IFA], 1:10,000 for western blotting [WB]; 66008-4-Ig, Proteintech), anti-HA polyclonal antibody (1:100 for IFA, 1:1,000 for WB; 51064-2-AP, Proteintech), anti-β-actin monoclonal antibody (1:10,000 for WB; 66009-1-Ig, Proteintech), anti-Cal nexin polyclonal antibody (1:200 for IFA; 10427-2-AP, Proteintech), anti-Syntaxin 6 polyclonal antibody (1:200 for IFA; 10841-1-AP, Proteintech), anti-Flotillin 1 monoclonal antibody (1:100 for IFA; 67968-1-Ig, Proteintech), and anti-Filamin A (FLNA) monoclonal antibody (1:1,000 for WB; 67133-1-Ig, Proteintech). .. Secondary antibodies included Alexa Fluor 488- or 568-conjugated goat anti-mouse/rabbit IgG (H+L) highly cross-adsorbed antibodies (1:1,000; Invitrogen), Wheat Germ Agglutinin Alexa Fluor Plus 488 (1:1000; W11261, Invitrogen), and HRP-conjugated goat anti-rabbit and anti-mouse IgG (1:5,000 for WB; 31,460 and 62-6520, Thermo Fisher).

    Article Title: Glycosylated NS3/NS3A protein of bluetongue virus facilitates efficient viral egress via lipid raft anchoring
    Article Snippet: .. Commercial antibodies used in this study included anti-FLAG (DYKDDDDK) monoclonal antibody (1:1,000 for immunofluorescence assay [IFA], 1:10,000 for western blotting [WB]; 66008-4-Ig, Proteintech), anti-HA polyclonal antibody (1:100 for IFA, 1:1,000 for WB; 51064-2-AP, Proteintech), anti-β-actin monoclonal antibody (1:10,000 for WB; 66009-1-Ig, Proteintech), anti-Calnexin polyclonal antibody (1:200 for IFA; 10427-2-AP, Proteintech), anti-Syntaxin 6 polyclonal antibody (1:200 for IFA; 10841-1-AP, Proteintech), anti-Flotillin 1 monoclonal antibody (1:100 for IFA; 67968-1-Ig, Proteintech), and anti-Filamin A (FLNA) monoclonal antibody (1:1,000 for WB; 67133-1-Ig, Proteintech). .. Secondary antibodies included Alexa Fluor 488- or 568-conjugated goat anti-mouse/rabbit IgG (H+L) highly cross-adsorbed antibodies (1:1,000; Invitrogen), Wheat Germ Agglutinin Alexa Fluor Plus 488 (1:1000; W11261 , Invitrogen), and HRP-conjugated goat anti-rabbit and anti-mouse IgG (1:5,000 for WB; 31,460 and 62-6520, Thermo Fisher).

    Western Blot:

    Article Title: Glycosylated NS3/NS3A protein of bluetongue virus facilitates efficient viral egress via lipid raft anchoring.
    Article Snippet: .. Commercial antibodies used in this study included anti-FLAG (DYKDDDDK) monoclonal antibody (1:1,000 for immuno fluorescence assay [IFA], 1:10,000 for western blotting [WB]; 66008-4-Ig, Proteintech), anti-HA polyclonal antibody (1:100 for IFA, 1:1,000 for WB; 51064-2-AP, Proteintech), anti-β-actin monoclonal antibody (1:10,000 for WB; 66009-1-Ig, Proteintech), anti-Cal nexin polyclonal antibody (1:200 for IFA; 10427-2-AP, Proteintech), anti-Syntaxin 6 polyclonal antibody (1:200 for IFA; 10841-1-AP, Proteintech), anti-Flotillin 1 monoclonal antibody (1:100 for IFA; 67968-1-Ig, Proteintech), and anti-Filamin A (FLNA) monoclonal antibody (1:1,000 for WB; 67133-1-Ig, Proteintech). .. Secondary antibodies included Alexa Fluor 488- or 568-conjugated goat anti-mouse/rabbit IgG (H+L) highly cross-adsorbed antibodies (1:1,000; Invitrogen), Wheat Germ Agglutinin Alexa Fluor Plus 488 (1:1000; W11261, Invitrogen), and HRP-conjugated goat anti-rabbit and anti-mouse IgG (1:5,000 for WB; 31,460 and 62-6520, Thermo Fisher).

    Article Title: Ubiquitously expressed transcript isoform 2 (UXT-V2) restricts HSV-2 replication by targeting glycoprotein B for degradation through ubiquitin-proteasome pathway
    Article Snippet: .. The commercial antibodies used in this study include: anti-Flag monoclonal antibody (1:5000 for immunoblotting, Sigma, St. Louis, MO; F3165), anti-His monoclonal antibody (1:2500 for immunoblotting, Sigma A7058), anti-HA polyclonal antibody (1:5000 for immunoblotting, Proteintech Group, Wuhan, China; S1064), anti-STING monoclonal antibody (1:2000 for immunoblotting, Cell Signaling Technology, Massachusetts, USA; #13647), anti-Ub polyclonal antibody (1:1000 for immunoblotting, Cell Signaling Technology 3936S), anti-UXT-V2 monoclonal antibody (1:1000 for immunoblotting, Sigma QC16645 and 1:1000 for immunoblotting, Mreda M198774), anti-TRIM21 antibody (1:2000 for immunoblotting, Proteintech Group, Wuhan, China; 83530-3-RR), anti-HSV1 + HSV2 gB antibody (1:2000 for immunoblotting, Abcam ab6506), and anti-HSV ICP5 Major Capsid Protein antibody (1:3000 for immunoblotting, Abcam Ab6508). ..

    Article Title: Uropathogenic Escherichia coli invade luminal prostate cells via FimH-PPAP receptor binding.
    Article Snippet: Cells were collected at 48 h post transfection in lysis buffer containing 1% NP-40, 5% glycerol, 25 mM Tris, 150 mM NaCl, 1 mM EDTA and protease inhibitor cocktail (05892791001, Sigma-Aldrich). .. Cell lysis was completed by incubation on ice for 1 h, followed by centrifugation at 15,000 × g for 1 h. Pellets were discarded and PPAP expression was examined by western blot using anti-HA polyclonal antibody (51064-2-AP, Proteintech, 1:5,000) and the secondary anti-rabbit IgG-HRP conjugate (GENA934, Sigma-Aldrich, 1:10,000). ..

    Article Title: Uropathogenic Escherichia coli invade luminal prostate cells via FimH–PPAP receptor binding
    Article Snippet: Cells were collected at 48 h post transfection in lysis buffer containing 1% NP-40, 5% glycerol, 25 mM Tris, 150 mM NaCl, 1 mM EDTA and protease inhibitor cocktail (05892791001, Sigma-Aldrich). .. Cell lysis was completed by incubation on ice for 1 h, followed by centrifugation at 15,000 × g for 1 h. Pellets were discarded and PPAP expression was examined by western blot using anti-HA polyclonal antibody (51064-2-AP, Proteintech, 1:5,000) and the secondary anti-rabbit IgG-HRP conjugate (GENA934, Sigma-Aldrich, 1:10,000). ..

    Article Title: Glycosylated NS3/NS3A protein of bluetongue virus facilitates efficient viral egress via lipid raft anchoring
    Article Snippet: .. Commercial antibodies used in this study included anti-FLAG (DYKDDDDK) monoclonal antibody (1:1,000 for immunofluorescence assay [IFA], 1:10,000 for western blotting [WB]; 66008-4-Ig, Proteintech), anti-HA polyclonal antibody (1:100 for IFA, 1:1,000 for WB; 51064-2-AP, Proteintech), anti-β-actin monoclonal antibody (1:10,000 for WB; 66009-1-Ig, Proteintech), anti-Calnexin polyclonal antibody (1:200 for IFA; 10427-2-AP, Proteintech), anti-Syntaxin 6 polyclonal antibody (1:200 for IFA; 10841-1-AP, Proteintech), anti-Flotillin 1 monoclonal antibody (1:100 for IFA; 67968-1-Ig, Proteintech), and anti-Filamin A (FLNA) monoclonal antibody (1:1,000 for WB; 67133-1-Ig, Proteintech). .. Secondary antibodies included Alexa Fluor 488- or 568-conjugated goat anti-mouse/rabbit IgG (H+L) highly cross-adsorbed antibodies (1:1,000; Invitrogen), Wheat Germ Agglutinin Alexa Fluor Plus 488 (1:1000; W11261 , Invitrogen), and HRP-conjugated goat anti-rabbit and anti-mouse IgG (1:5,000 for WB; 31,460 and 62-6520, Thermo Fisher).

    Lysis:

    Article Title: Uropathogenic Escherichia coli invade luminal prostate cells via FimH-PPAP receptor binding.
    Article Snippet: Cells were collected at 48 h post transfection in lysis buffer containing 1% NP-40, 5% glycerol, 25 mM Tris, 150 mM NaCl, 1 mM EDTA and protease inhibitor cocktail (05892791001, Sigma-Aldrich). .. Cell lysis was completed by incubation on ice for 1 h, followed by centrifugation at 15,000 × g for 1 h. Pellets were discarded and PPAP expression was examined by western blot using anti-HA polyclonal antibody (51064-2-AP, Proteintech, 1:5,000) and the secondary anti-rabbit IgG-HRP conjugate (GENA934, Sigma-Aldrich, 1:10,000). ..

    Article Title: Uropathogenic Escherichia coli invade luminal prostate cells via FimH–PPAP receptor binding
    Article Snippet: Cells were collected at 48 h post transfection in lysis buffer containing 1% NP-40, 5% glycerol, 25 mM Tris, 150 mM NaCl, 1 mM EDTA and protease inhibitor cocktail (05892791001, Sigma-Aldrich). .. Cell lysis was completed by incubation on ice for 1 h, followed by centrifugation at 15,000 × g for 1 h. Pellets were discarded and PPAP expression was examined by western blot using anti-HA polyclonal antibody (51064-2-AP, Proteintech, 1:5,000) and the secondary anti-rabbit IgG-HRP conjugate (GENA934, Sigma-Aldrich, 1:10,000). ..

    Incubation:

    Article Title: Uropathogenic Escherichia coli invade luminal prostate cells via FimH-PPAP receptor binding.
    Article Snippet: Cells were collected at 48 h post transfection in lysis buffer containing 1% NP-40, 5% glycerol, 25 mM Tris, 150 mM NaCl, 1 mM EDTA and protease inhibitor cocktail (05892791001, Sigma-Aldrich). .. Cell lysis was completed by incubation on ice for 1 h, followed by centrifugation at 15,000 × g for 1 h. Pellets were discarded and PPAP expression was examined by western blot using anti-HA polyclonal antibody (51064-2-AP, Proteintech, 1:5,000) and the secondary anti-rabbit IgG-HRP conjugate (GENA934, Sigma-Aldrich, 1:10,000). ..

    Article Title: Uropathogenic Escherichia coli invade luminal prostate cells via FimH–PPAP receptor binding
    Article Snippet: Cells were collected at 48 h post transfection in lysis buffer containing 1% NP-40, 5% glycerol, 25 mM Tris, 150 mM NaCl, 1 mM EDTA and protease inhibitor cocktail (05892791001, Sigma-Aldrich). .. Cell lysis was completed by incubation on ice for 1 h, followed by centrifugation at 15,000 × g for 1 h. Pellets were discarded and PPAP expression was examined by western blot using anti-HA polyclonal antibody (51064-2-AP, Proteintech, 1:5,000) and the secondary anti-rabbit IgG-HRP conjugate (GENA934, Sigma-Aldrich, 1:10,000). ..

    Centrifugation:

    Article Title: Uropathogenic Escherichia coli invade luminal prostate cells via FimH-PPAP receptor binding.
    Article Snippet: Cells were collected at 48 h post transfection in lysis buffer containing 1% NP-40, 5% glycerol, 25 mM Tris, 150 mM NaCl, 1 mM EDTA and protease inhibitor cocktail (05892791001, Sigma-Aldrich). .. Cell lysis was completed by incubation on ice for 1 h, followed by centrifugation at 15,000 × g for 1 h. Pellets were discarded and PPAP expression was examined by western blot using anti-HA polyclonal antibody (51064-2-AP, Proteintech, 1:5,000) and the secondary anti-rabbit IgG-HRP conjugate (GENA934, Sigma-Aldrich, 1:10,000). ..

    Article Title: Uropathogenic Escherichia coli invade luminal prostate cells via FimH–PPAP receptor binding
    Article Snippet: Cells were collected at 48 h post transfection in lysis buffer containing 1% NP-40, 5% glycerol, 25 mM Tris, 150 mM NaCl, 1 mM EDTA and protease inhibitor cocktail (05892791001, Sigma-Aldrich). .. Cell lysis was completed by incubation on ice for 1 h, followed by centrifugation at 15,000 × g for 1 h. Pellets were discarded and PPAP expression was examined by western blot using anti-HA polyclonal antibody (51064-2-AP, Proteintech, 1:5,000) and the secondary anti-rabbit IgG-HRP conjugate (GENA934, Sigma-Aldrich, 1:10,000). ..

    Expressing:

    Article Title: Uropathogenic Escherichia coli invade luminal prostate cells via FimH-PPAP receptor binding.
    Article Snippet: Cells were collected at 48 h post transfection in lysis buffer containing 1% NP-40, 5% glycerol, 25 mM Tris, 150 mM NaCl, 1 mM EDTA and protease inhibitor cocktail (05892791001, Sigma-Aldrich). .. Cell lysis was completed by incubation on ice for 1 h, followed by centrifugation at 15,000 × g for 1 h. Pellets were discarded and PPAP expression was examined by western blot using anti-HA polyclonal antibody (51064-2-AP, Proteintech, 1:5,000) and the secondary anti-rabbit IgG-HRP conjugate (GENA934, Sigma-Aldrich, 1:10,000). ..

    Article Title: Uropathogenic Escherichia coli invade luminal prostate cells via FimH–PPAP receptor binding
    Article Snippet: Cells were collected at 48 h post transfection in lysis buffer containing 1% NP-40, 5% glycerol, 25 mM Tris, 150 mM NaCl, 1 mM EDTA and protease inhibitor cocktail (05892791001, Sigma-Aldrich). .. Cell lysis was completed by incubation on ice for 1 h, followed by centrifugation at 15,000 × g for 1 h. Pellets were discarded and PPAP expression was examined by western blot using anti-HA polyclonal antibody (51064-2-AP, Proteintech, 1:5,000) and the secondary anti-rabbit IgG-HRP conjugate (GENA934, Sigma-Aldrich, 1:10,000). ..



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